I’ve let it expire because I needed the cash to see my folks in the UK and I really haven’t the slightest interest in making money from it.
I do dispatch, I’m sure it’s of absolutely no use to me in this shed and it’s a useful research tool for a bunch of experiments that haven’t been done yet.
So I hope someone uses the idea one day, I really just registered it so people would know it was my thought bubble.
I wrote it while listening to Duran Duran and Madonna so I could be as mindless as possible to face it.
HERV and METHYLATION ACTIVITY RATIO TEST
High levels of endogenous retroviral activity are associated with autoimmune diseases, cancers and symptomatic AIDS while low levels of S-adenosylmethionine are also associated with autoimmune diseases, cancers and AIDS.
The HERV and Methylation Activity Ratio test (HAMAR) takes two very different markers of methylation to monitor methylation levels in a patient and enable a researcher or physician to decide whether interventions to correct methylation are needed.
Summary of Claims:
A method of research to measure and monitor two markers of methylation and create a diagnostic ratio;
measuring reverse transcriptase levels of all retroviral activity in a blood sample;c) measuring S-adenosylmethionine levels in a blood sample;
d) high levels of reverse transcriptase and low S-adenosylmethionine levels correlating with global hypomethylation in a patient;
e) low levels of reverse transcriptase and higher S-adenosylmethionine levels correlating with normal methylation levels in a patient.
The method of claim wherein a patient has an autoimmune disease, has cancer or AIDS.
The method of claim being a measurement of a blood sample.
A method of measuring changing methylation levels in a patient and monitoring progress by;
obtaining blood samples over time from a patient;c) measuring levels of reverse transcriptase in said blood sample;
d) measuring levels of S-adenosylmethionine in said blood sample;
e) high levels of reverse transcriptase and low S-adenosylmethionine levels correlating with global hypomethylation in a patient;
f) low levels of reverse transcriptase and higher S-adenosylmethionine levels correlating with normal methylation levels in a patient.
A method of monitoring the effect of drug therapies on methylation levels and to diagnose and measure the effect of any methylation therapies used to correct abnormal methylation levels.
BACKGROUND OF THE INVENTION
[1] HERV-K is an endogenous retrovirus that appears in Autoimmune diseases, Cancer and also AIDS.
Detection of HERV-K(HML-2) viral RNA in plasma of HIV type 1-infected individuals.
AIDS Res Hum Retroviruses. 2006 Oct;22(10):979-84
[2] Other endogenous retroviruses are also expressed in autoimmune diseases such as Arthritis, Multiple Sclerosis and Psoriasis.
New Retrovirus Sequence Found in Psoriasis Lesions
Br J Dermatol. 2005 Jul;153(1):83-9
[3] Global hypomethylation of the genome is seen as a cause of retroviral transcription and methylation as a way to control expression of HERV-K and other retroviruses.
CpG methylation directly regulates transcriptional activity of the human endogenous retrovirus family
HERV-K(HML-2 J Virol. 2005 Jan;79(2):876-83
[4]Methylation has been also suggested as a therapy for cancer, autoimmune diseases and AIDS.
Methylation Therapy Dr. Timothy Bestor
Biomedical Frontiers: Winter/Spring 1996, Vol.3, No.2
[5] S-adenosylmethionine is a vital component of cellular methylation processes and low levels can be predictive of patient progress or worsening of symptoms in autoimmune diseases, cancers and AIDS.
Blood Determinations of S-Adenosylmethionine, S-Adenosylhomocysteine, and Homocysteine Cancer Epidemiology Biomarkers & Prevention
Vol. 10, 649-655, June 2001
[6] Removing the precursor nutrients for S-adenosylmethionine synthesis (choline, methionine, folic acid, and vitamin B12 ) creates spontaneous tumors in rats with subsequent global hypomethylation of the genome and an increase in retrotransposon activity.
LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period.
J Biomed Biotechnol. 2006; 2006: 17142
[7] S-adenosylmethionine can also be of therapeutic value and has been used as a safe treatment in diseases.
Safety and efficacy of S-adenosylmethionine (SAMe) for osteoarthritis.
J Fam Pract. 2002 May;51(5):425-30
[8] Adequate levels of Glutathione in the cells also influence the synthesis of S-adenosylmethionine and affect subsequent levels of SAMe
HIV infection: effect of parenteral treatment with SAMe
Neurology. 1995 Sep;45(9):1678-83.
[9] Adequate levels of S-adenosylmethionine prevent hypomethylation of DNA.
The methyl donor S-adenosylmethionine inhibits active demethylation of DNA; a candidate novel mechanism for the pharmacological effects of S-adenosylmethionine
J. Biol. Chem, 10.1074
[10] Infection with Pneumocystis carinii pneumonia lowers S-adenosylmethionine concentrations and can therefore become a potential cause of DNA hypomethylation and retroviral expression.
S-adenosylmethionine concentrations in diagnosis of Pneumocystis carinii pneumonia
Lancet Volume 361, Issue 9365, 12 April 2003, Pages 1267-1268
[11] Infection with tuberculosis can lower glutathione levels and can therefore become a cause of hypomethylation and retroviral expression.
Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects
AIDS Res Ther. 2006; 3: 5.
[12] Drug therapies can deplete cellular glutathione supplies and other methyl donors such as folic acid creating the conditions for global hypomethylation of the genome and subsequent retroviral expression.
Roles of endogenous ascorbate and glutathione in the cellular reduction and cytotoxicity of sulfamethoxazole-nitroso
Toxicology Volume 222, Issues 1-2, 1 May 2006, Pages 25-36
[13] S-adenosylmethionine can be used safely as a part of drug therapy to prevent liver damage and also maintain proper levels of cellular methylation.
S-Adenosylmethionine (AdoMet) supplementation for treatment of chemotherapy-induced Liver injury
Anticancer Res. 2003 Nov-Dec;23(6D):5173-9
[14] Exposure to chemicals, drugs, radiation, viruses and bacteria and inadequate methylation nutrients in the diet can all affect supplies of glutathione and S-adenosylmethionine creating global hypomethylation.
The HAMAR test can be used to measure and monitor these global hypomethylation affects and monitor if any retroviral expression is created by DNA hypomethylation.
[15] There is a need for research to answer the question how much do methylation compounds such as S-adenosylmethionine or the nutritional precursors for methylation affect DNA hypomethylation levels?
Also do deficiencies of these methylation nutrients cause hypomethylation and subsequent retroviral expression in cancer, autoimmune disease and AIDS?
B.A. May said it precisely “The replication of human immunodeficiency virus (HIV) may be modulated in part by host factors such as DNA methylation.
Hypermethylation of the HIV provirus may suppress viral replication and play a role in the establishment of latency. HIV seropositive individuals have decreased levels of metabolites involved in methylation.
It is proposed that metabolites such as S-adenosylmethionine (SAM), methylcobalamin and methyltetrahydrofolate be explored as potential therapeutic agents in HIV infected individuals.” A novel antiviral strategy for HIV infection.
Med-Hypotheses. 1993 Feb; 40(2): 93-4
Some drugs such as AZT appear to affect retroviral transcription by creating global DNA hypermethylation and hence suppressing retroviral expression.
AZT and other drugs can deplete Glutathione and SAMe.
How useful Glutathione and SAMe administration is in correcting ‘decreased levels of metabolites involved in methylation’ while also suppressing retroviral expression with or without other therapies requires further research.
The HAMAR test is a potential research tool.
DESCRIPTION OF THE INVENTION
The HERV and Methylation Activity Ratio test utilizes two types of test for two different methylation markers to monitor methylation changes in a patient.
The first part of the test is for measuring levels of the retroviral enzyme reverse transcriptase which is used by all retroviruses exogenous and endogenous.
An efficient and useful test for this is the PERT
PERT (Product-Enhanced Reverse Transcriptase) assay
(Pyra et al. PNAS 1994;91:1544-8; U.S. patent nr.5'807'669; EU patent nr.0623176).
By measuring for all levels of reverse transcriptase this avoids the need for specific retroviral detection.
Global DNA hypomethylation encourages expression of both endogenous and exogenous retroviruses.
The total of all retroviral reverse transcriptase levels then becomes a useful marker of DNA hypomethylation.
The inventors of PERT, Pyra H, Böni J, and Schüpbach J. describe it fully by saying the PERT assay permits detection of all retrovirus particles exhibiting an enzymatically active RT at a diagnostic sensitivity which is equal to that of specific viral RNA detection by PCR and has the additional advantage of not being negatively affected by sequence
variability.
One of the uses of the PERT test is for screening of human or animal disorders (cancer, autoimmune & other chronic diseases) for the possible involvement of exogenous or
endogenous retroviruses.
The PERT will pick up endogenous retroviruses that function in the placenta so may not be valid with pregnant patients.
The PERT assay for active reverse transcriptase can be used as a marker of global hypomethylation in a patient.
The other part of the HERV and Methylation Activity Ratio test measures levels of S-adenosylmethionine (SAMe) as another marker of methylation in a patient.
In a research setting the test used by Stepan Melnyk, Marta Pogribna, Igor P. Pogribny, Ping Yi and S. Jill James may be the most accurate for use at the moment.
Measurement of Plasma and Intracellular S-Adenosylmethionine and S-Adenosylhomocysteine
(Clinical Chem. 2000;46:265-272.)
This also has the advantage of monitoring levels of S- adenosylhomocysteine which can lower the levels of S- adenosylmethionine causing hypomethylation.
In a clinical setting there is a patented test for measuring levels of S-adenosylmethionine in blood.
Facile Diagnosis and Monitoring of Pneumocystis Carinii Infection
United States Patent 20050032035
Patented by Salim Merali.
This test measures S-adenosylmethionine levels as a marker of PCP infection, low S-adenosylmethionine levels correlating with worsening infection with PCP.
Measuring S-adenosylmethionine levels can also become a diagnostic marker of methylation changes in cancer, autoimmune diseases and AIDS.
There are different methods of measuring SAMe and different methods can be used as methylation markers.
EXAMPLE OF THE INVENTION
If any patient is infected with Pneumocystis Carinii Infection then PCP uses up the cellular supplies of S-adenosylmethionine (SAMe) and this can cause DNA hypomethylation creating the possible conditions for endogenous retroviral expression or increased HIV.
The preferred drug for PCP treatment is Bactrim or Sulfamethoxazole which can create Glutathione deficiency and is also a possible risk factor for hypomethylation.
If the patient is undergoing other drug therapies this can also create further deficiencies in Glutathione which can reduce SAMe supplies creating potential hypomethylation and
retroviral expression, endogenous or exogenous.
The levels of endogenous HERV-K retrovirus can rise in parallel with HIV, the PERT assay is a useful marker of all these active retroviral reverse transcriptase (RT) levels.
RT is a potential marker of global DNA hypomethylation if measured in cancers, autoimmune diseases as well as AIDS.
By measuring SAMe levels a physician can also monitor whether drug therapies being used affect the SAMe levels and whether to intervene with SAMe administration or
the nutrients needed for correct methylation.
RT and SAMe levels are measured at the beginning of therapy, during therapy and preferably post therapy.
Any changes in these markers of methylation then becomes a useful research or clinical tool for monitoring patient outcomes and progress while undertaking different therapies.
Increased RT and decreasing SAMe would indicate global hypomethylation in a patient and symptoms as a result.
Decreased RT and preferably normal SAMe levels being the measured outcome indicating patient progress with diseases.
The claims defining the invention are as follows:
[1] A method of research to measure and monitor two markers of methylation and create a diagnostic ratio;
measuring reverse transcriptase levels of all retroviral activity in a blood sample;c) measuring S-adenosylmethionine levels in a blood sample;
d) high levels of reverse transcriptase and low S-adenosylmethionine levels correlating with global hypomethylation in a patient;
e) low levels of reverse transcriptase and higher S-adenosylmethionine levels correlating with normal methylation levels in a patient.
[2] The method of claim 1 wherein a patient has an autoimmune disease, has cancer or AIDS.
[3] The method of claim 1 and 2 wherein the measurement is from a blood sample.
[4] A method of measuring changing methylation levels in a patient and monitoring progress by;
obtaining blood samples over time from a patient;c) measuring levels of reverse transcriptase in said blood sample over time;
d) measuring levels of S-adenosylmethionine in said blood sample over time;
e) high levels of reverse transcriptase and low S-adenosylmethionine levels correlating with global hypomethylation in a patient;
f) low levels of reverse transcriptase and higher S-adenosylmethionine levels correlating with normal methylation levels in a patient.
[5] The method of claim 4 wherein a patient has an autoimmune disease, has cancer or AIDS.
[6] The method of claim 4 and 5 wherein the measurement taken over time is from a blood sample.
[7] A method of monitoring the effect of drug therapies on methylation levels and to measure the effect of any methylation therapies used to correct abnormal methylation.
[8] The method of claim 7 wherein a patient has an autoimmune disease, has cancer or AIDS.
[9] The method of claim 7 and 8 wherein the measurement is from a blood sample.
[10] A method of detemining whether nutritional intervention with SAMe or SAMe methylation precursors affects DNA hypomethylation and the potential for retroviral expression from either endogenous or exogenous retroviruses.
[11] The method of claim 10 wherein a patient has an autoimmune disease, has cancer or AIDS.
[12] The method of claim 10 and 11 wherein the measurement is from a blood sample.
22nd August 2007
ABSTRACT
The HERV and Methylation Activity Ratio Test uses two different tests to measure levels of retroviral reverse transcriptase enzyme and S-Adenosylmethionine in the blood of a patient over time.
These can be used as markers of methylation to create a diagnostic ratio that can monitor or correct DNA methylation levels in patients suffering from cancer, autoimmune diseases or AIDS.
Well, I obviously don’t believe HIV causes AIDS and this patent couldn’t have been written if I did.
I believe global hypomethylation of DNA is a major cause of many diseases and the only appropriate treatment is to monitor a patient’s diet and make sure they have the essential nutrients needed to maintain methylation and stability of their DNA.
If this makes me a heretic then that’s your problem, you need to think about this mystery a wee bit more if you want to see people stay alive.
I also don’t believe HIV is an infectious retrovirus.
I think the PCR test picks up a racially specific sequence of endogenous retrovirus.
Kary Mullis the inventor of the PCRdoesn’t believe HIV causes AIDS because the levels of retrovirus that his test amplifies are so small and not specific they couldn’t possibly be pathogens.
Not specific in that they say there are two strains of HIV but the reality is that not one single HIV sequence is the same as the other.
And that’s why I believe the sequences are racially specific retroviruses that are only coming out of the genome of people either starved of methylation nutrients or exposed to chemicals, drugs and other infections that cause hypomethylation.
If you go back to the beginning of this fiasco you have to ask questions.
How did Robert Gallo get his antibody test approved when only one third of his AIDS patients were positive to the HIV antibody test?
What caused AIDS in the other two thirds?
Robert Gallo cultured ‘HIV’ on umbilical cords.
Umbilical cords are full of retroviruses because retroviruses are needed for fetal implantation to the placenta.
Why were the concerns about popper use, drug and chemical exposure never researched fully?
The ELISA antibody test in conjunction with the Western Blot test is supposed to be 99% accurate but when you look a bit further they say you can be false positive…..
“Pregnancy. If this is not her first pregnancy, a woman may react positively when she is, in fact, negative.
History of injection drug use.
Cross-reactivity with other retroviruses.”
Why do the manufacturers have disclaimers?
Legally any doctor who tells you that you have HIV/AIDS is lying to you and if you are labeled as HIV positive then you have every right to litigate against your doctor……..and Robert Gallo.
This is because the test manufacturers have covered themselves by saying this is just a blood-screening test for the presence of retroviruses.
None of it is specific.
It takes less than an hour to Google the antigens used in these tests and you will find them in all the diseases where retroviruses have increased due to global hypomethylation.
Cancers and all the autoimmune diseases.
This means if you are sick with a cancer or arthritis, psoriasis, MS, hyperthyroid you are in danger of testing positive.
Around 75% of all of us are supposed to have antigens to the p24 of the bovine leukemia retrovirus.
Again this is not a retrovirus that causes leukemia in cattle but an endogeous cow retrovirus that appears because of the global hypomethylation that occurs in leukemias.
So this is why they have to dilute the HIV test 400 times.
If we applied the HIV test at 1 to 1 we’d all be positive.
Why do they say pregnant women can be positive with the test, it’s not 99% accurate then is it?
The reason why pregnant mums test positive is because the test cross reacts with other retroviruses and we now know that retroviruses like HERV-W are jumping all around the growing babies as the cells undergo hypomethylation and methylation with each division.
So what do we see in the children exposed to AIDS drugs?
Their growth is stunted, they get nerve and brain damage because methylation is vital for nerve development, some get mutations and never make it.
Some simply get poisoned to death before all this happens.
Of course we say we’re curing them because we’ve stopped all the retroviral activity.
Dead.
Why has there never been peer review of HIV/AIDS as with all other theories?
Why has Nature magazine been so terrified of allowing Peter Duesberg his right of reply?
…..For a quarter of a century.
Why do scientists presume that gays, drug users and blacks are at high risk of contracting AIDS?
This is obvious bigotry gone mad but no one can see it?
It’s obvious to me why gays, drug users and blacks get AIDS.
Gays are party boys, many do take too many drugs, that’s ok if you keep it under control but when AIDS hit the partying was pretty hard, it broke their bodies.
It’s still happening, drug users are the same.
And the resulting exposure to too many drugs is depleting their nutrients that are needed to keep their retroviruses in check, their genomes are hypomethylating and that’s why they test positive.
Africans are just starving and dying from TB and their genomes are hypomethylating too.
We all expected HIV/AIDS to run rampant through prostitutes but that didn’t happen, mainly drug using prostitutes were getting the syndrome.
Why do prostitutes not get AIDS?
While pregnant women do?
It’s very simple.
They get way more cash than the average person in the third world, this means they get to eat proper food.
So they eat enough nutrients to maintain methylation of their DNA and no retroviruses appear for the AIDS scientists to measure.
Of course if we told alcoholics they were at risk of HIV and needed to get tested again and again there would be an imaginary HIV epidemic amongst alcoholics.
Why can’t you see these things?
Racism is even built into the test.
If antibodies to the HLA-DR African genes cross react with the p24 antigen then this explains why more blacks are HIV positive.
If you have antibodies to HLA-DR then it probably means you have an autoimmune disease and of course your endogenous retroviruses will be coming out because your DNA is hypomethylating.
But it’s not AIDS caused by HIV.
Why did no one censure Robert Gallo for suggesting that Africans eating chimp meat were infected with chimp retroviruses and then somehow took HIV to Haiti?
Then the gays who went on sex tours to Haiti got infected and brought HIV back to America.
These are the murmurings of a madman, why are you fans of him?
How on earth do you expect people to get well when you give them poisons?
This is really Munchausen syndrome by proxy on a planetary scale as we con people into taking poisons to keep them sick.
This is a saddening thing for me to write about, it’s not fun.
I just found this other history of our world and kept looking, it’s been 11 years in the wilderness, I’ve only met one AIDS dissident who was a lovely HIV tagged guy.
When I had coffee at Fat Louie’s with him I was the one who was sick from Benzene exposure.
He looked fine, I presume he’s still fine, he knew the score on AIDS drugs.
Avoid them and you live to a ripe old age.
Other HIV positive friends who have had the support of family to walk away from this are alive.
But the harrowing stories I’ve been told have traumatized me.
This is not a science.
This is an inquisition.
The worst thing is the bullying, intimidation and terrorizing of HIV positives into taking AIDS drugs, if you don’t take them everyone will shun you.
If you do then you get sick anyway.
I’ve seen stories from friends like one lady who came up positive when pregnant, then was forced to abort, then retested and came up negative.
I was swapping emails with her as it happened.
Only recently I was told one lady’s story about being shunned by not just her community but her family as well.
Bullied into taking the drugs, put under immense pressure to drug her children, she’s stopped medication but it sounds like the drugs have done too much damage to her organs.
All I could imagine is this lovely women isolated and stuck indoors with a community willing her to die.
I’m worried because I haven’t heard from her.
We are using modern day voodoo on people.
The T-cell test is the main voodoo machine we use.
First we tell people that they have a deadly retrovirus that WILL kill them.
This creates incredible fear, bone pointing and psychic attack are real in the world of AIDS.
When you get afraid your body pumps out Cortisol.
If Cortisol levels rise and stay that way then the result is depletion of T-cells.
Fear of death actually reduces your immunity in reality.
It’s well documented.
You just don’t want to face reality.
Now anyone who’s smug about this should know what is coming up for the HIV gang this century, I know it’s as good as over but you don’t.
It’s the children.
They aren’t all dying like you thought they would.
It’s been standard practice with the AIDS establishment to recruit several thousand children for pediatric drug trials, we have no idea what they are doing off shore and in other countries.
We have entire hospitals full of so called orphan kids.
In America the majority are kids removed from drug addicted Black or Hispanic mothers.
They get experimented on, they get poisoned and many die.
These tough little sprites aren’t all dying.
Somehow there’s a spark of life in some of them that keeps them going even as the AZT
When they get to the magic age of 18 they get away from the g-tubes, walk out of the orphanages and say stuff you to any doctor stupid enough to try and tell them what to do.
When they get out they are free.
I look at it as like being a concentration camp survivor.
So you need to be scared of them because they will have a score to settle.
I’m saying this because if you do work in HIV/AIDS you still have time to jump off the bandwagon.
The litigation will take time but when it does start running.
As for me, I looked, I worked out exactly what all this data means so I’m happy.
You can believe what you want but I’ll think you’re mad and you have no comeback.
I’m off to play music and join the theatre.
Might even go home and cry, kind of used to that.
I'll be back to talk about cancer properly soon and then I'll have this horrible stuff out of my head.
Have a lovely century everyone.
From me...
Cal Crilly
Star Sludge